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NOTICE: This avoids the coupling of amino acids within the additional cycles with the membrane instead of the growing peptide chain. Make certain all the purification buffers are at 4 °C and carry out the protein purification within the cold room to keep away from protein denaturation. BE AWARE: After the coupling of the first C-terminal amino acid of all peptides to the amino group of the membrane, the amino teams between the spots and in addition a few of the amino teams inside the spot areas don't form bonds with the amino acids. |
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