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+NOTICE: This avoids the coupling of amino acids within the further cycles with the membrane as an alternative of the growing peptide chain. Be sure that all the purification buffers are at 4 °C and carry out the protein purification within the cold room to keep away from protein denaturation. NOTICE: After the coupling of the primary C-terminal amino acid of all peptides to the amino group of the membrane, the amino teams between the spots and in addition a number of the amino teams throughout the spot areas don't form bonds with the amino acids.
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